Deoxyribonuclease I, DNase I
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DNase I digests double- and single-stranded DNA into oligo- and mononucleotides.Using a buffer, DNA is removed from RNA in a quick and simple digestion. The DNase I is then heated with a solution to induce inactivation. Heating also denatures hairpins in the RNA, so the RNA can be used directly in reverse transcription. No current RNA isolation procedure removes 100% of the DNA. Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription.
DNase I (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5’-phosphorylated and 3’-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.
Recombinant enzyme. Purified from non-animal host with a lower level of intrinsic RNase
– Degradation of DNA template in transcription reactions.
– Removal of contaminating genomic DNA from RNA samples.
– DNase I footprinting.
– Nick Translation
100 mg, 50 mg, 500 mg