Cell Culture
Specialized cell types and stem cells are sensitive to their environment
Stem cells and other specialized cell types are especially sensitive to their environment. Minimize risk by using Akron reagents, either research or
Growth conditions for culturing stem cells must be optimized in order to maintain the cells’ phenotypes and biological activities. The composition of the medium and extracellular matrix on which the stem cells are maintained need to closely resemble that of the cells in the undifferentiated state. Product specifications will vary with the requirements of the particular type of stem cell you’re working with. So will the type of culture medium supplements your cells need to maintain optimum growth, and to stabilize self-renewable capacity.
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Albumin
Albumin is the most abundant protein in serum, and it has many applications and functions in cell culture. It increases cell health, growth, and productivity.
Transferrin
Transferrin is an important component in growth media. It is a glycoprotein, but also known more specifically as an iron-storage protein found in mammalian serum (i.e. a blood plasma protein). Transferrin receptors on the cell surface of actively growing cells bind transferrin for iron support to and from cells.
Culture Medium
In order to grow, cultured cells need an environment full of nutrients that is simultaneously sterile, with stable temperatures and acidity. Culture medium are such environments, and foster cell growth for scientific purposes with excellent results.
Culture Medium Supplements
Culture medium supplements are added to the culture medium and contain extra nutrients in order to provide for optimum cell growth.
Extracellullar Matrix
Extracellular matrices (ECM) are diverse in nature and composition; they can serve many functions, such as providing support, segregating tissues from one another, and regulating intercellular communication. Formation of the ECM is essential for processes like growth, wound healing and fibrosis. An understanding of the ECM structure and composition also helps in comprehending the complex dynamics of cell proliferation.
3D Matrix
3D cell culture is Akron Biotech's new range of 3D cell culture scaffolds made from electrospun polymeric nanofibers. Make your cells in culture feel right at home by giving them a substrate that closely resembles the mechanical and physical features of natural extracellular matrix.
Cell Dissociation
Cell dissociation is the removal of adherent cells from the surface of a culture. It typically requires another substance, which is used to complete the removal.
Cryopreservation
Cryopreservation is necessary for the long term storage of stem cells. It is crucial to provide long-term storage conditions that maintain and undifferentiated state, stable karyotype, revival viability and ability to proliferate.
Cell Assays
Cell Assays are used to determine cell count and cell viability within a cell sample.
