Recombinant Human Platelet Derived Growth Factor BB (E.coli)
Cat # Product Name Size
CYT-PBB002
CYT-PBB010
CYT-PBB01M		 Human Platelet Derived Growth Factor-BB 2ug
10ug
1mg

Synonyms: Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide, Becaplermin.

Description: rh Platelet-Derived Growth Factor BB is a homodimeric, non-glycosylated, polypeptide chain containing 109 amino acids.

Molecular mass: 24,300 Da

Formulation: The protein was lyophilized without additives.

Purity and Identification: by proprietary chromatographic techniques.

SDS-PAGE: Not more than 3% total contaminants

RP-HPLC: principal peak>97%

Protein Content: Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280nm using the absorbancy value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard.

Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined to be Ser-Leu-Gly-Ser-Leu.

Biological Activity: The ED50 calculated by the dose-dependent proliferation of BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1 ng/ml.

Specific Activity: 1 x 10(6) IU/mg.

Source: E. coli

Physical State of the product: Sterile filtered white lyophilized (freeze-dried) powder.

Solubility: It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in 100mM acetic acid.

Stability and Storage: Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA)and store below -18°C.

Please avoid freeze-thaw cycles.

Customized sizes upon request.

Usage: For research use only. Not for use in diagnostics or therapeutic procedures.

To place an order, please contact our Sales Department at sales@akronbiotech.com