Human Recombinant Interleukin-2 (concentrated solution)
Cat # Product Name Size
CYT-IL2010
CYT-IL2050
CYT-IL201M 		Human Interleukin 2 10 ug
50 ug
1mg

Formulation: rh IL-2; Sodium Phosphate 10 mM; 0.22 mg SDS per mg of protein; pH = 7.5 - 8.0.

Purity and Identification: SDS-PAGE (reducing): Not more than 3% total contaminants

SDS-PAGE (non-reducing): Not more than 1% contaminants in the oligomeric region.

HPLC: principal peak>95%.

Western blot specific antibody identification.

Absence of host cell derived proteins: less than 0.6%; and host cell derived DNA (by dot blot hybridization): less than 100pg per mg of protein. Technical Reports Series N˚747-WHO.

Sterility Test: USP XXVIII

Safety Test: USP XXVIII

Endotoxin level: <25 EU per mg of protein. (LAL test).

Biological Activity: Determined on a cell proliferation assay using an IL-2 dependent T cell line, CTLL-2. (Gearing, A, Bird C.B. in Lymphokines and Interferons, a Practical Approach. 1987, M.J. Clemens, A.G. Morris and A.J.H. Gearing, eds. IRL Press, p296)

Specific activity: Not less than 10 MIU and not more than 22 MIU per mg of protein, according to lot #.

Molecular Mass: 15,000 +/- 1,000 Da

Source: E. coli

Physical State of the product: liquid, 2-8 ˚C

Shipping conditions: refrigerated 2-8 ˚C

Selected References: List of selected References for IL-2

Protein Databases: Q13169

Customized sizes upon request.

Usage: For research use only. Not for use in diagnostics or therapeutic procedures.

• Cell proliferation assay using an IL-2 dependent T cell line, CTLL-2. (Gearing, A, Bird C.B. in Lymphokines and Interferons, a Practical Approach. 1987, M.J. Clemens, A.G. Morris and A.J.H. Gearing, eds. IRL Press, p296)

• Analyzed on Graph Pad Prism 4 software

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