Human Recombinant G-CSF (concentrated solution)
Cat # Product Name Size
CYT-GCS002
CYT-GCS010
CYT-GCS01M 		Human Granulocyte-Colony Stimulating Factor 2 ug
10 ug
1mg

Formulation: rh G-CSF, Sodium Acetate 10 mM, Tween 80 0.04 mg/ml, pH 4.0-4.5.

Purity and Identification: SDS-PAGE (reducing): not more than 3% contaminants.

SDS-PAGE (non-reducing): not more than 3% contaminants in the oligomeric region.

HPLC: principal peak >95%

Western blot specific antibody identification.

Absence of host cell derived proteins: less than 0.6%; and host cell derived DNA (dot blot hybridization): less than 100 pg per 480 ug of protein. Technical Reports Series N˚747-WHO.

Sterility Test: USP XXVII

Safety Test: USP XXVII

Endotoxin level: <100 EU per mg of protein. (LAL test).

Biological Activity: Determined on a cell proliferation assay using an AML cell line, (Santoli D et al. J. of Immunol. 1987 139: 3348-3354)

Specific activity: Not less than 40 x 106 IU per mg, and not more than 160 x 106 IU per mg of protein, according to lot #.

Molecular Mass: 18,300 +/- 1,000 Da

Source: E. coli

Physical State of the product: liquid, 2-8 ˚C

Shipping conditions: refrigerated 2-8 ˚C

Selected References: List of selected References for G-CSF

Protein Databases: P09919

Customized sizes upon request.

Usage: For research use only. Not for use in diagnostics or therapeutic procedures.

• Cell proliferation assay using an AML cell line, (Santoli D et al. J. of Immunol. 1987 139: 3348-3354)

• Analyzed on Graph Pad Prism 4 software

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