Recombinant Human Fibroblast Growth Factor
Cat # Product Name Size
CYT-FGFB010
CYT-FGFB050
CYT-FGFB01M 		Human Fibroblast Growth Factor-basic 10ug
50ug
1mg

Synonyms: FGF b, FGF 2, Prostatropin, HBGH-2, HBGF-2

Description: rh Fibroblast Growth Factor-2 is a single, non-glycosylated, polypeptide chain containing 155 amino acids.

Molecular Mass: 17,154 Da. http://www.expasy.org/uniprot/P09038

Formulation: The protein is suspendedPhosphate-NaCI Buffer pH=7.4 it is a sterile solution

Purity and Identification: by proprietary chromatographic techniques.

SDS-PAGE: Not more than 2% total contaminants

RP-HPLC: principal peak>95%

Protein Content: Protein quantitation was carried out by fluoroscent method using Quant-it Protein Assay Kit and by A280 2.6mg/mL.

Coefficient Extinction: 0.929

Amino Acid Sequence: The sequence of the first five amino acids was determined to be Met-Ala-Ala-Gly-Ser

Biological Activity: The ED50 calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <1.5 ng/mL.

Source: E. coli

Physical State of the Product: Sterile filtered white lyophilized (freeze-dried) powder.

Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile nanopure water not less than 100µg/ml, which can then be further diluted in other aqueous solutions.

Stability and Storage: Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution bFGF should be stored at 4°C between 2-7 days. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) and store below -18°C.

Please avoid freeze-thaw cycles.

Customized sizes upon request.

Usage: For research use only. Not for use in diagnostics or therapeutic procedures.

To place an order, please contact our Sales Department at sales@akronbiotech.com