Recombinant Human Endostatin
Cat # Product Name Size
CYT-END020
CYT-END100
CYT-END01M 		Human Endostatin 20ug
100ug
1mg

Description: rh Endostatin is a single, glycosylated, polypeptide. It has been identified as the C-terminal fragment of Collagen type 18, and is a member of a family of collagen-like proteins referred to as multiplexin family. Endostatin specifically inhibits proliferation of endothelial cells although it does not affect the proliferation of EOMA cells.It has been shown to act as a potent inhibitor of angiogenesis and tumor growth in vitro and in vivo. It induces tyrosine phosphorylation of Shc (SH2 domain adapter protein) leading to specific inhibition in endothelial cell proliferation.

Molecular mass: 20,000 Da

Formulation: 5mg/ml in PBS.

Purity and Identification: by proprietary chromatographic techniques. Greater than 98.0% as determined by:

Analysis by RP-HPLC.

Anion-exchange FPLC.

Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: In total agreement with the expected amino acid composition of native human Endostatin.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Protein content: Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm.

2. Analysis by RP-HPLC, using a calibrated solution of Endostatin as a Reference Standard.

Physical Appearance: Sterile filtered liquid

Stability and Storage: Human Endostatin although stable at 4ºC for 30 days, should be stored desiccated below -20 ºC for periods greater than 30 days.

Please avoid freeze-thaw cycles.

Biological Activity: is fully biologically active when compared to standards. The activity calculated by ECE migration inhibition was found to be 50,000IU/mg.

Endotoxin: Less than 0.1 ng/µg (IEU/µg)

Source: Pichia pastoris

Customized sizes upon request.

Usage: For research use only. Not for use in diagnostics or therapeutic procedures.

To place an order, please contact our Sales Department at sales@akronbiotech.com