Human Recombinant Erythropoietin (concentrated solution)
Cat # Product Name Size
CYT-EPO500
CYT-EPO05K
CYT-EPO01M 		Human Erythropoietin 500 U
5000U
1mg

Formulation: rh EPO, 10 mM Sodium Phosphate, 26mM Sodium Chloride, pH 7.0-7.5

Purity and Identification: SDS-PAGE: single broad band of sample corresponds to EPO Reference Standard BRP(EDQM)

Isoelectric Focusing, Peptide Mapping and Western blot data correspond to Reference Standard BRP (EDQM).

HPLC: principal peak >98%.

Absence of host cell derived proteins: less than 0.6%; and host cell derived DNA (by dot blot hybridization): less than 100 pg per 40 ug of protein. Technical Reports Series N 747-WHO.

Sialic acid test: not less than 10 mol of sialic acid per mol of EPO.

Sterility Test: USP XXVII

Safety Test: USP XXVII

Endotoxin level: Not more than 20 EU per 100000 U. (LAL Test).

Biological Activity: Determined on a cell proliferation assay using the TF-1 cell line (Kitamura T,1989 J. of Cell. Physiol. 140: 323).

Specific activity: Not less than 100 000 IU per mg of protein, according to lot #.

Molecular Mass: approximately 37,000 Da

Source: CHO cells

Physical State of the product: frozen (-20˚C)

Shipping conditions: dry ice (<-20˚C)

Selected References: List of selected References for EPO

Protein Databases: P01588

Customized sizes upon request.

Usage: For research use only. Not for use in diagnostics or therapeutic procedures.

• Cell proliferation assay using the EPO dependent TF-1 cell line (Kitamura T,1989 J. of Cell. Physiol. 140: 323).

• Analyzed on Graph Pad Prism 4 software

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